分光光度计722说明书谁有?

722/722N/722S可见分光光度计  使用说明书      
            本说明书详细阐述了722/722N/722S可见分光光度计的使用方法、故障排除及维护与保养等内容。请在使用前仔细阅读本说明书，并将其随附仪器。    制作计量器具许可证编号:      沪制01120041号  
  
722  /  722N/722S可见分光光度计    目录    章节  标题  页码      1  原理、用途和特点··························································································  1  1.1  原理················································································································  1  1.2  用途················································································································  1  1.3  特点················································································································  1      2  仪器规格和标准附件·······················································································  1      3  仪器操作键和各部分名称、功能·····································································  2      4  安装指导·········································································································  4  4.1  安装条件·········································································································  4  4.2  开箱检视·········································································································  4      5  操作················································································································  4  5.1  仪器的基本操作······························································································  4  5.1.1  预热················································································································  4  5.1.2  改变波长·········································································································  4  5.1.3  校正波长准确度······························································································  5  5.1.4  置参比样品和待测样品···················································································  5  5.1.5  置0%(T)  ········································································································  5  5.1.6  置100%(T)  ····································································································  5  5.1.7  改变操作模式··································································································  6  5.1.8  浓度因子设定和浓度直读设定········································································  6  5.1.9  RS232C  串行接**换数据···········································································  6  5.2  应用操作·········································································································  7  5.2.1  测定透明材料的透射比···················································································  7  5.2.2  测定透明材料的T  –  λ(透射比-波长)曲线························································  7  5.2.3  测量透明溶液的吸光度···················································································  7  5.2.4  运用A-C(吸光度-浓度)标准曲线测定物质浓度················································  8  5.2.5  浓度直读应用··································································································  9  5.2.6  浓度因子功能应用··························································································  9      6  仪器维护和故障识别······················································································  10  6.1  仪器日常维护·································································································  10  6.1.1  使用注意事项·································································································  10  6.1.2  开罩检视  ······································································································  10  6.1.3  更换光源········································································································  10  6.2  常见故障识别及处理······················································································  11  
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1  原理、用途和特点：                                                                    
  
1.1  原理                                                                      可见分光光度计是一种结构简洁、使用方便的单光束分光光度计，基于样品对单色光的选择吸收特性可用于对样品进行定性和定量分析。其定量分析根据相对测量原理工作，即选定样品的溶剂(或空气)作为标准试样，设定其透射比为100%，被测样品的透射比则相对于标准试样(或空气)而得到，在一定的浓度范围，各参量遵循朗伯—比耳定律：
  0/1  lg  IITKCL  T  A===    A:  吸光度    T:  相对于标准试样的透射比    I:  光透过被测样品后照射到光电传感器上的强度    I0:  光透过标准试样后照射到光电传感器上的强度    K:  样品溶液的比消光系数    L:  样品溶液在光路中的长度    C:  样品浓度
  
1.2  用途                                                                      广泛应用于医药卫生、临床检测、生物化学、石油化工、环保监测、食品生产和质量控制等部门作定性、定量分析，还可作为大、专院校和中学相关课程的教学演示和实验仪器。
  
1.3  特点                                                                      波长和测试参量全数字显示    自动置0%(T)，自动置100%(T)透射比功能  浓度因子和浓度直读设定，浓度直读功能  标准RS232C串行接口和PC计算机通讯  
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  仪器规格及标准附件                                            光学系统:  C—T型单色仪，1200线/mm全息光栅  波长范围:  335  ～  1000nm  光    源:  卤钨灯  (20W  /  12V)  波长准确度:  ±2nm  波长重复性:  1nm  光度准确度:  ±0.5%(T)    光度重复性:  0.2%(T)  
  2      光谱带宽:  4nm    杂  散  光:  ≤  0.5%(T)  (在360nm处)    T-A转换误差:  ≤±0.002A(在0.5A处)；≤±0.004A(在1A处)；  显示标尺  T:  0  ～  199.9%    A:  -0.3  ～  2.5    F:  0  ～  9999    C:  0  ～  9999  供电电压:  220V±10%  50Hz    尺寸:  385(W)  ×  310(D)  ×  190(H)  mm  重量:  净重6.5Kg  标准配置:      主机一台    4个10mm光程普通光学玻璃矩形比色皿，1个黑体（用于挡光置零）    电源线一根    使用说明书一份    保险丝(T1A/250V)二只    
  
3  仪器操作键和各部名称、功能  (见仪器外形图)                    
  3.1  MODE键：用于选择操作模式。连续按下MODE键，按透射比、吸光度、浓度因子、浓度直读的工作次序，指示灯分别循环点亮，指示仪器当前的操作模式。  
      前视图
  
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      后视图    
  3.2  FUNC键：预定功能扩展键：浓度因子指示灯亮时，用于设定浓度因子时的数字移位；浓度直读指示灯亮时，用于设定浓度直读时的数字移位；在透射比、吸光度、浓度因子和浓度直读各操作模式下，用于将当前显示从RS232C串行口发送到PC机。
  3.3  100%ADJ键：当透射比指示灯亮时，按下一次，自动调整100%(T)(一次未到位可加按一次)；当吸光度指示灯亮时，仍作为100%(T)的设定键，显示吸光度值0.000；当浓度因子指示灯亮时，用于减小浓度因子的设定值；当浓度直读指示灯亮时，用于减小浓度直读的设定值。
  3.4  0％ADJ键：当透射比指示灯亮时，用作自动调整0%(T)(一次未到位可加按一次)；当吸光度指示灯亮时，该键不起作用；当浓度因子指示灯亮时，用于增加浓度因子的设定值；当浓度直读指示灯亮时，用于增加浓度直读的设定值。
  3.5  浓度直读指示灯：当指示灯亮时，指示仪器处于测量浓度和浓度直读的操作模式。
  3.6  浓度因子指示灯：当指示灯亮时，指示仪器处于设定浓度因子的操作模式。
  3.7  吸光度指示灯：当指示灯亮时，指示仪器处于测量吸光度的操作模式。
  3.8  透射比指示灯：当指示灯亮时，指示仪器处于测量透射比的操作模式。
  3.9  数值显示窗(4位LED数字)：显示测试值、出错信息和溢出信息。
  3.10  波长显示窗(4位LED数字)：显示波长值。
  3.11  样品室：供安装各种样品室附件用。
  3.12  波长调节手轮：改变波长用。
  3.13  样品架拉杆：用于改变样品架的位置(四位置)。
  3.14  RS232C串行接口插座：用于联接RS232C串行电缆实现和PC机联机工作。
  3.15  可变电阻器：用于精确校正波长准确度。
  3.16  电源插座：接插电源线。

1.接通电源，打开仪器开关，掀开样品室暗箱盖，预热10分钟。
　　2.将灵敏度开关调至“1”档（若零点调节器调不到“0”时，需选用较高档。）
　　3.根据所需波长转动波长选择钮。
　　4.将空白液及测定液分别倒入比色杯3/4处，用擦镜纸擦清外壁，放入样品室内，使空白管对准光路。
　　5.在暗箱盖开启状态下调节零点调节器，使读数盘指针指向t=0处。
　　6.盖上暗箱盖，调节“100”调节器，使空白管的t=100，指针稳定后逐步拉出样品滑竿，分别读出测定管的光密度值，并记录。
　　7.比色完毕，关上电源，取出比色皿洗净，样品室用软布或软纸擦净。
注意事项
　1．该仪器应放在干燥的房间内，使用时放置在坚固平稳的工作台上，室内照明不宜太强。热天时不能用电扇直接向仪器吹风，防止灯泡灯丝发亮不稳定。
　　2．使用本仪器前，使用者应该首先了解本仪器的结构和工作原理，以及各个操纵旋钮之功能。在未按通电源之前，应该对仪器的安全性能进行检查，电源接线应牢固，通电也要良好，各个调节旋钮的起始位置应该正确，然后再按通电源开关。
　　3．在仪器尚未接通电源时，电表指针必须于“0”刻线上，若不是这种情况，则可以用电表上的校正螺丝进行调节。

分光光度计722又称光谱仪(，是将成分复杂的光，分解为光谱线的科学仪器。测量范围一般包括波长范围为380~780  nm的可见光区和波长范围为200～380  nm的紫外光区。不同的光源都有其特有的发射光谱,因此可采用不同的发光体作为仪器的光源。钨灯的发射光谱：钨灯光源所发出的380～780nm波长的光谱光通过三棱镜折射后，可得到由红、橙、黄、绿、蓝、靛、紫组成的连续色谱；该色谱可作为可见光分光光度计的光源。